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Tocilizumab (Actemra) Pharmacokinetic ELISA Kit
货号:EL-1611-202 | 规格:96 wells | 价格:¥10508.00 | 品牌:AffinityImmuno
托珠单抗是一种免疫抑制性人源化单克隆抗体药物,主要用于治疗类风湿关节炎,系统性幼年特发性关节炎。Tocilizumab (Actemra) Pharmacokinetic ELISA Kit托珠单抗(Actemra)药代动力学ELISA试剂盒是由北京中昊新生科技有限公司提供。

托珠单抗(雅美罗)药代动力学ELISA试剂盒

Tocilizumab (Actemra®) is an immunosuppressive humanized monoclonal antibody drug, mainly for the treatment of rheumatoid arthritis (RA) and systemic juvenile idiopathic arthritis, a severe form of arthritis in children. Tocilizumab binds the interleukin-6 receptor (IL-6R) thus blocking the signaling caused by interleukin 6 (IL-6). IL-6 is a cytokine that plays an important role in immune response and is implicated in the pathogenesis of many diseases.


The Tocilizumab ELISA kit is designed to measure free Tocilizumab with high specificity and sensitivity.


PRINCIPLE OF THE ASSAY

This assay employs the sandwich enzyme immunoassay technique. Anti- Tocilizumab is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Tocilizumab present in biological matrices is bound by the immobilized anti- Tocilizumab antibody. After washing away any unbound substances, enzyme linked anti- Tocilizumab antibody is added to the wells. This antibody is developed and purified specifically against Actemra® (domain residing in Fc portion of the Actemra® molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Tocilizumab present in test samples. The color development is stopped and the intensity of the color is measured.

APPLICATION(S):measure free Tocilizumab

TARGET PROTEIN:actemra

ALTERNATIVE NAME OF TARGET:tocilizumab

SENSITIVITY:detection limit 1.5 ng/mL

DETECTION METHOD:Peroxidase / OD450

PRECISION:Inter-assay CV <10%.

DETECTION LIMIT:1.5ng/ml

STORAGE CONDITIONS:Stable at -20°C for 1 year

SAFETY WARNINGS AND PRECAUTIONS:Research Use Only. Follow instructions.

EACH KIT INCLUDES:

Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (500 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3


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