Ceramide glycanase is an enzyme used to release glycans from glycosphingolipids (GSLs) to enable their characterisation. It cleaves glycans including GM1, GM2 and GM3 by cleaving the β-glycosyl linkage. Glycans can then be labelled using LudgerTag labelling technology.

Application:
Ceramide glycanase can be used to deglycosylate a variety of glycosphingolipids by cleaving the β-glycosyl linkage.

Source: Hirudo medicinalis

Contents:
Ludger Ceramide Glycanase - Kit Contents
LudgerZyme Ceramide Glycanase - 1 vial of 55 µL
RXN buffer (4x concentrate) - 1 vial of 150 µL
GM1 glycolipid - 1 vial of 10 µL (approx. 2 nMol)

Number of Samples: Sufficient for up to 25 samples

Amount of Samples: Up to 1 nmol of glycolipid

Suitable Samples: Isolated glycolipids containing a ceramide backbone

Suggested usage:
1. Pipette 5 µL of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 µL of approximately 1nmol of the glycolipid sample to be tested.
2. For the positive control reaction, add 5 µL of GLIP-GM1-01 substrate to 5 µL of LZ-CER-BUFFX4 into an Eppendorf tube
3. Add 2 µL of LZ-CER-HM-10 (the enzyme) to the reaction mixture and mix gently by tapping the Eppendorf tube with your finger. Make total reaction mix up to 20 µL with water.
4. Incubate your samples in a water bath, oven or any other constant heating source at 37°C for 24 hrs.

The samples are now ready for procainamide, 2-AB or 2-AA labeling and subsequent analysis by HPLC.
Samples can be stored frozen at this point.

Storage:
Long term storage - over 14 days - store at – 20°C.
Protect from sources of heat and light. Avoid multiple freeze thaw cycles.

Short term storage - up to 14 days - store at 4°C.
Protect from sources of heat and light.