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Ludger Liberate Orela O-glycan release kit
货号:LL-ORELA-A2 | 规格:12 Samples | 价格:¥7936.00 | 品牌:Ludger
LudgerLiberate Orela O糖释放试剂盒,The Ludger Liberate™ Orela Kit has been developed for O-linked deglycosylation of glycoproteins. Released glycans have free reducing terminii to allow fluorescent tagging by reductive amination.
The kit includes reagents and materials for O-linked deglycosylation of up to 12 glycoprotein samples analysed in parallel or two sets of 6 samples, typically up to 1 mg of glycoprotein per sample. This method of O-glycan release demonstrates significant reduction in peeling and reduces the number of sample handling steps prior to release.

– Designed for easy, O-linked deglycosylation by QC labs.
– Straightforward and simple method.
– Released glycans have free reducing terminii suitable for fluorescent labelling prior to analysis by HPLC and MS.
– Fluorescent labelling allows relative quantitation of glycan species by HPLC.
– Faster and lower cost than hydrazinolysis (LL-HYDRAZ-A2).
– No special handling techniques.

The Orela Reaction
The Orela reagent reacts at the link between the glycan and peptide backbone to liberate glycans as the glycosylamine. The acid-labile glycosylamine derivative is hydrolysed to produce the free glycan.

Outline of the Orela Protocol
Prepare the glycoconjugate
Prepare the glycoprotein or glycopeptide samples by removing contaminants such as salts, detergents and dyes that could interfere with the labeling procedure.

Dry the glycans
Place the samples in reaction vials and dry down.

Add release reagent to glycoconjugates
Add Orela release reagent to each sample.

Incubate
Incubate the samples to allow the release reaction to progress.

Acidification
Add acetic acid solution and incubate at 4C to convert glycosylamines into non-reduced glycans

Post-release cleanup
Remove the peptide or protein using a cation exchange column

Store or Analyse released glycans
The released glycans are now ready for analysis
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