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抗氧化还原能力检测试剂盒
货号:NWK-ARC02 | 规格:100 tests | 价格:¥0.00 | 品牌:Northwest Life Science
抗氧化还原能力检测试剂盒,灵敏度30 mM Trolox 等效物,定量检测血清,血浆,细胞培养物,食品提取物和其他水性生物样品中抗氧化状态,

NWLSS™ Antioxidant Reductive Capacity (Antioxidant Status) Assay Kit

NWLSS™ 抗氧化能力试剂盒测量样品将Cu++还原为Cu+的能力,作为评估抗氧化状态的一种手段。该试剂盒可作为一种简单的方法来量化多种水样(包括血清、血浆、细胞培养基和食品提取物)的通用抗氧化能力。

◆ 不适用于含有EDTA或其他金属螯合剂的样品。


Advantages of the NWLSS™ Antioxidant Reductive Capacity Assay:

◆ Simple protocol returns data for antixidant status in 5 minutes.

◆ Data may be reported as Troloxor Uric Acid Equivalents.

◆ Trolox standards provided for easy comparison to ORAC data.


Oxidative stress is defined as a condition that exists when there is a lack of balance between pro-oxidants and antioxidants. Oxidative stress appears to be involved in the pathogenesis of several diseases including artherosclerosis, chronic inflammatory disease and cancer. Since oxidative stress can be harmful to organisms, intra-cellular, extra-cellular, enzymatic and non-enzymatic systems exist to mediate the condition. Lipid soluble antioxidants (most importantly vitamin E) and water-soluble antioxidants (uric acid, vitamin C, bilirubin, thiols and gluthathione) are a few of the antioxidants involved in these processes. Given the large number of antioxidant pathways, and their importance in regulation of an organism's redox status, it is important to be able to quantitatively estimate the capacity of antioxidants to reduce or antioxidant power within biological specimens.


Test Principle

The NWLSS™ Antioxidant Reductive Capacity Assay is based on the combined action of sample antioxidants to reduce Cu++ to Cu+. Cu+ reacts with bathocuproine (BC) to form a color complex with maximal absorbance at 480-490 nm. Measurement at 490 nm before and after addition of BC generates a net absorbance in direct relation to a sample's reductive capacity. Absorbance values obtained for samples are compared with a standard curve generated using Trolox. Alternatively, researchers may select a different standard of their choosing for use in the assay.


格式:100 Test,比色;

用途:血清、血浆、细胞培养物、食品提取物和其他水性生物样品中抗氧化状态的定量测量;

样品要求:血清、血浆、组织和其他生物样品(不推荐用于EDTA血浆样品或可能存在金属螯合剂的其他样品);

特异性:特异性测定抗氧化还原能力生物样品;

灵敏度:30 mM Trolox当量;

储存和稳定性:12个月(2-8℃)。


Kit Contents:

1 X 60 mL Dilution Buffer containing bathocuprione disulfonic acid.

1 X 5 mL Cu++ solution)

1 X 5 mL Stop Solution containing EDTA.

1 Vial Trolox Standard

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