NWLSS™ 谷胱甘肽还原酶检测试剂盒是一种基于速率的动力学检测，用于定量检测包括血细胞、组织和培养细胞在内的各种样品中的谷胱甘肽还原酶 (GR)。

Glutathione Reductase is a ubiquitous 100-120 kD dimeric flavoprotein that catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) using b-nicotinamide dinucleotide phosphate (NADPH) as the hydrogen donor. A measurable fraction of GR derived from various sources is often found as the inactive apoenzyme. Dietary supplementation of riboflavin or thyroxin has been shown to activate GR. The in vitro addition of FAD is the basis of assessing riboflavin deficiency. The active site for GR contains a flavin adenine dinucleotide (FAD) and a disulfide. In the presence of NADPH, there is a two electron reduction of GR to produce a semiquinone of FAD, a sulfur radical and a thiol. Purified GR tends to form aggregates in the absence of thiols and these aggregates retain full enzymatic activity. Purified enzyme is reversibly inactivated by NADPH, m-nicotinamide adenine dinucleotide (NADH), GSH, dithionite or borohydride. This inactivation likely requires the presence of divalent cations such as Zn⁺⁺ and Cd⁺⁺. The GR enzyme is fully protected from inactivation by ethylenediamine tetraacetic acid (EDTA). Inactivated GR is activated by GSSG, NADP⁺ and NAD⁺. In vivo, GR activity is regulated through a redox interconversion mechanism mediated by GSSG regulation of the NADPH generating pathways.

格式：192 well 微孔板（2块板）
用途：定量生物样品中的GR酶活性，如组织匀浆和细胞裂解液

样品要求：组织和细胞裂解液

特异性：谷胱甘肽还原酶活性

灵敏度：LLD = 1.5 mU/mL

储存和稳定性：按规定储存，9个月

Kit Contents:

NADPH Reagent 

NADPH Diluent 

GSSG Reagent 

GR Calibrator Assay 

Buffer Microplates