The Ludger EC50 plate has been designed to purify glycans from non-carbohydrate material including salts, proteins and detergents by electronic interaction of the glycans with the surface of the cartridge.

• Removes salts, detergents, and proteins to enhance HPLC and mass spec analysis
• Easy to use
• Improves the signal intensity after labeling

96-well Ludger EC50 plates can be used:
• After enzymatic or chemical release of glycans from glycoproteins
• After exoglycosidase (enzymatic) digestion of glycans to release individual monosaccharides to confirm glycan identity and structure
• Before and after glycan labelling using fluorescent tags such as 2-aminobenzamide acid (2-AB) or 2-aminobenzoic acid (2-AA)

The 96-well Ludger EC50 plates contain an electron interaction resin (EIR). This unique material contains very flat regions of pi-orbital electrons that interact with hydrophilic organic compounds including glycans.

The resin can capture a wide range of glycans from complex mixtures while salts, proteins, and certain detergents pass through. The glycans are then eluted from the cartridge using a simple solvent system.

2-AB Labeling Efficiency: Use of EC50 Cartridges vs No Clean-up