蛋白激酶A活性检测试剂盒(比色)

DetectX®PKA(Protein Kinase A)活性检测试剂盒用于定量检测各种样品中的PKA活性。

样本类型:Cell Lysates, Purified Systems

Time to Answer:3小时

Samples/Kit:42 in Duplicate

稳定性:Fully Active PKA Standard Stored at -20ºC

读数:Colorimetric, 450 nm

DetectX®Protein Kinase A(PKA)活性检测试剂盒提供一种灵敏而可靠的方法，用于筛选PKA的抑制剂或活化剂以及在在多种样品类型中定量测定PKA活性。96孔格式试剂盒使用合成肽作为PKA的底物，并使用兔单克隆抗体通过3小时的分析识别底物的磷酸化形式。在进行操作前请阅读完整试剂盒说明书或咨询中昊新生(zhscience.com)。

随着测定方法的改进，该试剂盒现在包含一种改进后的激酶反应缓冲液配方和更灵敏的标准范围，而不需要两个波长读出。试剂盒整体性能的改进，使检测灵敏度提高了10倍。 灵敏度的提高导致标准曲线范围和试剂制备的改变。 新范围将允许使用更简单的检测方案测量样品中较低浓度的PKA。

                    
The expressed PKA holoenzyme, comprising of two catalytic (C) and two regulatory (R) subunits, is activated when cAMP levels rise following stimulation of G Protein-coupled receptors and adenylyl cyclase. The phosphorylation of specific substrates by the C subunit of activated PKA is regulated by the subcellular localization of the enzyme through the binding to the scaffolding A kinase-anchoring proteins (AKAPs). In its inactive state, the pseudosubstrate sequences on the R subunits stop the activity of the C subunits. Upon binding of cAMP to the R subunits, the active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC. Substrates that are phosphorylated by PKA include Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK -3β Ser9). PKA is a pivotal kinase involved in cancer, vasodilation, metabolic processes, etc.

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Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。
北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供过Protein Kinase A(PKA)活性检测试剂盒,蛋白激酶A活性试剂盒最新现货优惠信息。