DetectX®BCA蛋白检测试剂盒用于定量检测各种样品中的总蛋白含量。

检测类型:Detection Kit

样本类型:Serum, Plasma, Urine, Cell Lysates, Tissue Homogenates

灵敏度:1.68 µg/mL

适应物种:所有物种

检测时间:2小时

样品数/板:41 in Duplicate

读数:Colorimetric, 560 nm

DetectX^®BCA蛋白双范围检测试剂盒提供检测样品蛋白含量所需的全部试剂，试剂盒可检测所有物种的所有类型的蛋白。执行试剂盒操作前请阅读详细说明书或咨询中昊新生(zhscience.com)。试剂盒包含牛血清白蛋白（BSA）标准品以生成测定的标准曲线，所有样品都应从标准曲线中读取。将样品在水中稀释并添加到孔中。BCA显色液由BCA试剂与BCA增强剂混合而成。将BCA显色溶液添加到所有孔中，37℃下孵育板。样品中的蛋白与BCA显色剂反应生成紫色产物，在560 nm处读取。

Protein determination is one of the most common operations performed in biochemical research. The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry assay, and relies on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to protein present. It has been shown that cysteine, cystine, tryptophan, tyrosine, and peptide bonds are able to reduce Cu2+ to Cu1+. BCA forms a purple-blue complex with Cu1+ in alkaline environments, thus providing a basis to monitor the reduction of alkaline Cu2+ by proteins.

The kit provides everything needed to measure protein content of a sample and the assay measures all types of proteins from all species. A bovine serum albumin (BSA) standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in water and added to the wells. The BCA Color Solution is made by mixing the BCA Reagent with the BCA Enhancer. The BCA Color Solution is added to all wells and the plate incubated at 37°C. Protein in the samples reacts with the BCA Color Reagent to generate a purple colored product which is read at 560 nm.

_______________________________________________

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。
北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供人BCA蛋白检测试剂盒最新现货优惠信息。