hGH ADA (human growth hormone) ELISA Kit

适用物种:Human

样本类型:Serum,Plasma

检测范围:30 ng/mL -1920 ng/mL

最低检出限:30 ng/mL

测定时间:2.5 hours

检测格式:Direct sandwich ELISA

检测原理:
This assay employs the indirect enzyme immunoassay technique. hGH is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to hGH present in biological matrices are bound to the immobilized hGH. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to hGH present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.

检测方法:Peroxidase / OD450 

靶抗原:antibodies to hGH

应用:Quantification of antibodies to hGH

包被板类型:Strip

样品体积:15ul

特异性:anti-hGH antibodies

检测精度(批内差异,批间差异):<20%, <20%

防腐剂:None

试剂盒组分:
Coated microtiter plate, 96 wells (12x8 strips) 1
Calibrator diluent 1.8 ml
Calibrator (1.15mg/mL) 12 μl
10X wash buffer 50 ml
Assay buffer 50 ml
1000X detection reagent 17 μl
TMB 12 ml
TMB stop solution

储存温度和稳定性:Stable at -20°C for 1 year

背景描述:
The AffinityImmuno hGH (Human Growth Hormone) ADA ELISA kit contains the components required for measuring antibodies to hGH in biological matrices such as human serum, plasma or cell culture supernatant.

检测程序:
This assay employs the indirect enzyme immunoassay technique. hGH is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to hGH present in biological matrices are bound to the immobilized hGH. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to hGH present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured

样本收集:
This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.

样品制备:
Dilute calibrators and test samples 1/100 with assay buffer (for example add 5µL of prepared calibrator or sample to 495µL of assay buffer). Mix well. Do not store diluted samples.

试剂制备:
Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label.
1. Wash Buffer (1X) Preparation Dilute wash buffer concentrate with deionized water 1/10 before use (for example add 20mL concentrate to 180mL deionized water). Mix well.
2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11μl concentrate to 11ml of assay buffer). Mix well.

结果计算:
1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. Alternatively a log-log curve fit may be used.
2. The concentration of the unknowns can be read directly from this standard curve using the absorbance value for each sample.
3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.



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