ABSbio™人VEGF ELISA试剂盒用于定量检测天然或重组人VEGF。试剂盒试剂足以在ELISA板中检测96孔人VEGF。

适用样品：血清，血浆和细胞培养基
检测范围：5-800 pg/ml
别名：VEGFA; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor; Vascular Endothelial Growth Factor A; 血管内皮生长因子; 血管内皮生长因子A

Vascular endothelial growth factor C is a VEGF. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis an endothelial cell growth and survival, and can also affect the permeability of blood vessels.

ABSbio™ Human VEGF ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human VEGF in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Human VEGF containing samples or standards and a biotinylated detection antibody directed against Human VEGF are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human VEGF in samples.

The kit is designed to quantitatively measure natural and/or recombinant Human VEGF within the range of 5–800 pg/ml. The kit is sufficient to assay human VEGF in approximately 96 ELISA plate wells. 

试剂盒组分