ABSbio™小鼠IL-10 ELISA试剂盒/小鼠白介素10 ELISA试剂盒,用于以夹心ELISA形式定量检测天然或重组小鼠IL-10,检测范围为23-2000 pg/ml,适用血清,血浆和细胞培养基样品。

IL-10 (Interleukin-10), also known as Mouse cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine.

ABSbio™ Mouse IL-10 ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Mouse IL-10 in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for highthroughput users. Mouse IL-10 containing samples or standards and a biotinylated detection antibody directed against Mouse IL-10 are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-10 in samples.

The kit is designed to quantitatively measure natural and/or recombinant Mouse IL-10 within the range of 23–2000 pg/ml. The kit is sufficient to assay Mouse IL-10 in approximately 96 ELISA plate wells.