鸡白介素2 ELISA试剂盒旨在以夹心ELISA形式定量检测样品中的鸡IL-2含量,检测范围47–3000 pg/ml。
This is a shorter ELISA assay that reduces time to 50% compared to the conventional method,and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for chicken IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for chicken IL-2 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the
wells and color develops in proportion to the amount of IL-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Storage:
Store at 4 °C. The kit can be used in 3 months.
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