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Active rabbit PAI-1 functional assay ELISA kit
货号:RbPAIKT | 规格:1 Kit | 价格:¥0.00 | 品牌:Molecular Innovations
Active rabbit PAI-1 functional assay ELISA kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1669、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of functionally active rabbit PAI-1 in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration of PAI-1 activity was reported to be 9.8 ng/ml in rabbit plasma and 2.9 ng/ml in rabbit serum. The assay measures active PAI-1 in the 0.05-50 ng/ml range. Samples giving rabbit PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. After appropriate washing steps, anti-rabbit PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.



Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody
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