Sulfo-SMCC crosslinker (Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate) is a water soluble, heterobifunctional crosslinker (SMCC is the water insoluble protein crosslinking analog of Sulfo-SMCC crosslinker). As such, the non-membrane piercing Sulfo-SMCC protein crosslinking reagent can conjugate extracellular proteins.

Sulfo-SMCC crosslinker has an 8.3 angstrom, non-cleavable spacer arm. Sulfo-SMCC conjugation occurs via a maleimide group which reacts with sulfhydryls (thiols, -SH), while its Sulfo-NHS ester is reactive toward primary amines (as found in Lysine and the protein or peptide N-terminus). Sulfo-SMCC crosslinking reagent is routinely used to prepare hapten-carrier protein, polymers, and antibody-enzyme conjugates, such as HRP conjugation.

SATA Protein Modifier can be used to add sulfhydryl groups (thiols, -SH) to proteins or peptides prior to Sulfo-SMCC crosslinking.

Molecular Weight: 436.37

Spacer Length: 8.3 Å

CAS Number: 92921-24-9

Chemical Formula: C16H17N2NaO9S

Alternative Names: 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester; Water Soluble SMCC; Sulfo-SMCC X-linker; SSMCC; S-SMCC; Sulfosuccinimidyl-4-N-maleimidomethyl-cyclohexane-1-carboxylate; Sulfo SMCC