统一服务热线:400-888-0207 在线咨询 | 微信联系
首页 > 产品 > 产品类型 > 试剂 > 样本制备
PolyFect Transfection Reagent
货号:301105 | 规格:1 ml | 价格:¥0.00 | 品牌:QIAGEN
PolyFect Transfection Reagent(QIAGEN 301105)采用先进的活化树状多聚物,从多聚物中心向外辐射出带正电荷的氨基基团与DNA 分子结合,为下列细胞株特别优化:COS-7、NIH/3T3、HeLa、293、CHO。

快速简单的DNA 转染,用于标准细胞株
For 25–65 transfections in 60 mm dishes or 50–100 transfections in 6-well plates.


Features

优化的细胞特异性转染方案

操作简便迅速

细胞存活率高,毒性低

Product Details

PolyFect Transfection Reagent采用先进的活化树状多聚物,从多聚物中心向外辐射出带正电荷的氨基基团与DNA 分子结合,为下列细胞株特别优化:COS-7、NIH/3T3、HeLa、293、CHO。


Performance

PolyFect Transfection Reagent保持高效转染率。与其他试剂不同的是,PolyFect Transfection Reagent能够在血清存在的情况下进行转染而不降低转染效率。

Principle

PolyFect Transfection Reagent是一种特殊活化的树状聚合物。它由从中心发散出分支的球形大分子构成。分支终止于带电的氨基,可与带相反电荷的磷酸基相互作用。PolyFect Reagent将DNA聚集成紧密结构,附于细胞表面通过非特异性内吞作用进入细胞。该试剂调节细胞核内pH,抑制核内核酸,从而保证了PolyFect–DNA复合物的稳定性。


Procedure

PolyFect Transfection Reagent是即用型试剂。转染流程简便迅速,只需要将试剂加入到DNA溶液中混合,反应5–10分钟,加入培养基(可以含血清和抗体),将PolyFect–DNA复合物移到细胞上。培养细胞用于基因表达。无需转染后移除复合物或更换及增加培养基,操作简便快速。


高通量转染

使用PolyFect Transfection Reagent进行转染只需少量手动操作且无需移除转染复合物,特别适用于高通量筛选。PolyFect Reagent在高通量转染中转染效率高,可重复性好,细胞毒性低,适用于批量转染。关于在96孔板中对COS-7、NIH/3T3、HeLa、HeLa-S3、293和CHO细胞进行转染的实验方案,请咨询QIAGEN技术支持。


Applications

PolyFect Transfection Reagent特别适用于标准细胞系高效转染,主要用于:


基因表达与功能研究

药物研究与开发


Supporting data and figures

High transfection efficiencies in CHO and 293 cells.

Transfection efficiencies for PolyFect Reagent and 2 lipid-based reagents were compared. Cells cultured in 6-well plates were transfected with a beta-galactosidase-reporter plasmid. The appropriate protocol was used for transfection with PolyFect Reagent. Optimized protocols were used for transfection with Reagents L2 and F; these were: 10 µl Reagent L2 with 3 µg DNA and 15 µl Reagent F with 2 µg DNA for CHO cells, and 10 µl Reagent L2 with 2 µg DNA and 7 µl Reagent F with 3 µg DNA for 293 cells. All transfections were performed in triplicate. Two independent transfection experiments are shown for 293 cells.

1_5 (1).jpeg

Specifications

Features                                         Specifications

Applications                                 RNAi studies, gene expression studies...

Transfection type                         Transient transfection, stable transfection, plasmid co-transfection

Controls                                         Not included

Features                                         Optimized protocols for COS-7, NIH/3T3, HeLa 293, and CHO cells. 

                                                        Transfection in the presence of serum

Technology                                 Activated dendrimers

Number of possible transfections 25–10,000 transfections

Nucleic acid                                 DNA

Cell type                                         COS-7, NIH/3T3, HeLa, 293, CHO cells


相关产品
相关资料