CRF (Corticotropin Releasing Factor) Antibody
The ImmunoStar antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat median eminence using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1000 – 1/2000 in PBS/0.3% Triton X-100 – Biotin/Avidin-HRP Technique.
Immuno-labeling is completely abolished by preadsorption with synthetic CRF at 100 ug per mL of diluted antibody.
照片描述: IHC image of the rat median eminence staining for CRF. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.
规格/体积: 100 µL
性状: Lyophilized Whole Serum
反应性: Mouse, Rat, Salmon, Turtle
别名: Corticoliberin; Corticotropin-Releasing Hormone,Endorpholiberin; corticotropin releasing hormone, anti-CRF
免疫原: Ovine CRF
Gene Symbol: CRH