The antibody has a proven strong biotin-streptavidin/HRP staining at 1/1,000-1/1,200 dilution in rat globus pallidus and amygdala.
Staining is completely eliminated by pretreatment with 100 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 100 µg of leucine enkephalin only partially blocks staining.
照片描述: High magnification of the globus pallidus with nickel preparation (above) and low magnification IHC image of methionine encephalin staining in the globus pallidus and amygdala for the rat brain (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
规格/体积: 100 µL
性状: Lyophilized Whole Serum
反应性: Astyanax Jordani (Fish), Bird, Blowfly, Bovine, Bullfrog, Cat, Chick, Chicken, Cockroach, Cod, Crab, Dog, Fish, Frog, Gerbil, Goldfish, Guinea Pig, Hamster, Human, Lamprey, Leech, Lymnaea Stagnalis (Snail), Manducta Sexta (Moth), Monkey, Mouse, Octopus, Parrot, Pig, Pigeon, Rabbit, Rana Esculenta (Frog), Rana Pipiens (Leopard Frog), Rana Temporaria (Frog), Rat, Ratfish, Salamander, Salmon, Sea Bass (Fish), Shrew, Squirrel Monkey, Starling, Tapeworm, Turtle, Zebra Finch
别名: Enkephalin (Met); Proenkephalin-A; Met-enkephalin; Opioid growth factor; OGF, anti-Methionine Enkephalin, Met Enk
Gene Symbol: PENK