葡萄糖摄取检测试剂盒

Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders such as myocardial ischemia, diabetes mellitus, and cancer. Screening for glucose uptake inhibitor is a new way to search for cancer treatments. The ABSbioTM AGlucose Uptake Assay Kit provides a simple, sensitive, fluorometric assay to evaluate the nonmetabolized glucose analog-2-deoxy-D-glucose (2DG) uptake in cultured cell. In this assay, 2-DG is taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). The amount of the accumulated non-metabolizable 2-DG6P is directly proportional to glucose uptake by cells. The accumulated 2-DG6P is enzymatically coupled to generate NADPH, which is specifically monitored by a NADPH sensor at Ex/Em=530/590 nm. The kit is supplied with sufficient reagents for 100 tests in 96-well plate assay, linear detection range of 0.05-10 µM 2-DG6P use 50 µL sample volume. It could easily be modified for use in 384-well assay and high-throughput assay. It can be used for the high throughput screening of glucose uptake regulators and inhibitors.

试剂盒组分

储存及处理: Shipping on icepack. Store kit at -20°C. Shelf life: 12 months after receipt. Warm Reagents to room temperature before use.

应用:Measurement of Glucose Uptake for a variety of cell types.

样品类型:Cell lysates

检测方法:Fluorescence (Ex/Em = 530/590 nm)

检出限:0.05-10 µM
灵敏度: The Limit of Detection was determined as 0.05 µM, and linear detection range up to 10 µM in 96-well plate assay.
Sensitivity was determined as 0.05 µM.Samples with values above 10 µM should be dilute with assay buffer, re-assayed, and multiply results by dilution factor.

反应时间:60 min

储存:-20°C

保质期:12 months after receipt

运输:icepack