过氧化氢检测试剂盒(荧光)

DetectX®Hydrogen Peroxide(H2O2)荧光检测试剂盒用于定量检测各种样品中的H2O2。

检测类型:Detection Kit

样本类型:Urine, Buffer, Tissue Culture Media

灵敏度:0.038 µM

适应物种:所有物种

检测时间:15分钟

样品数/板:40 in duplicate

读数:Fluorescent, 590 nm Emission / 520 nm Excitation

DetectX®过氧化氢荧光检测试剂盒提供过氧化氢标准品以生成标准曲线。将样品与荧光底物混合，并加入辣根过氧化物酶来引发反应，反应在室温下孵育15分钟。HRP在过氧化氢的存在下与底物反应，将无色底物转化为荧光产物。荧光产物在590 nm处读取，在570 nm处激发。H2O2水平的增加导致荧光产物呈线性增加。

In biological systems, incomplete reduction of O2  during respiration produces superoxide anion (O2 -·), which is spontaneously or enzymatically dismutated by superoxide dismutase to H2O2 . Many cells produce low levels of O2 -· and H2O2 in response to a variety of extracellular stimuli, such as cytokines (TGF-β1, TNF-α, and various interleukins), peptide growth factors (PDGF, EGF, VEGF, bFGF, and insulin), the agonists of heterotrimeric G protein–coupled receptors (GPCR) such as angiotensin II, thrombin, lysophosphatidic acid, sphingosine 1-phosphate, histamine, and bradykinin, and by shear stress. The addition of exogenous H2O2 , or the intracellular production in response to receptor stimulation, affects the function of various proteins including protein kinases, protein phosphatases, transcription factors, phospholipases, ion channels, and G proteins. In 1894, Fenton described the oxidation of tartaric acid by Fe2+ and H2O2. H2O2  and O2  may participate in the production of singlet oxygen and peroxynitrite and the generation of these species may be concurrent with reactions involving iron, which under some circumstances might be important contributors to H2O2 toxicity.

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Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。
北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供过氧化氢检测试剂盒,Hydrogen Peroxide Fluorescent Assay Kit最新现货优惠信息。