ABSbio™ 大鼠VEGF ELISA试剂盒/大鼠血管内皮生长因子ELISA试剂盒,用于以夹心ELISA形式定量检测天然或重组大鼠VEGF,检测范围为23-2000 pg/ml,适用样品:血清,血浆及细胞培养基。

Vascular endothelial growth factor C is a VEGF. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels.

ABSbio™ Rat VEGF ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Rat VEGF in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Rat VEGF containing samples or standards and a biotinylated detection antibody directed against Rat VEGF are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat VEGF in samples. The kit is designed to quantitatively measure natural and/or recombinant Rat VEGF within the range of 23–2000 pg/ml. The kit is sufficient to assay Rat VEGF in approximately 96 ELISA plate wells.