ABSbio™大鼠IL-2 ELISA试剂盒/大鼠白介素2 ELISA试剂盒,用于以夹心ELISA形式定量检测天然或重组大鼠IL-2,检测范围为5–1000 pg/ml,适用样品:血清,血浆及细胞培养基。

IL-2 (Interleukin-2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells.

ABSbio™ Rat IL-2 ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Rat IL-2 in serum, plasma & cell culture media. The strip plate is coated with the antibody and preblocked to provide timesaving for high-throughput users. Rat IL-2 containing samples or standards and a biotinylated detection antibody directed against Rat IL-2 are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IL-2 in samples. The kit is designed to quantitatively measure natural and/or recombinant Rat IL-2 within the range of 5–1000 pg/ml. The kit is sufficient to assay Rat IL-2 in approximately 96 ELISA plate wells.