鸡白介素6 ELISA试剂盒旨在以夹心ELISA形式定量检测样品中的鸡IL-6含量,检测范围1–4500 pg/ml。
This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 4 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for chicken IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for chicken IL-6 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Storage:
Store at 4 °C. The kit can be used in 3 months.