LudgerTag 2-AB多糖标记试剂盒(2PB 还原剂),2-AB (2-aminobenzamide) is one of the most widely used fluorescent labels for glycosylation analysis. The LudgerTag 2-AB Labeling Kit includes all the reagents required to label up to 30 glycans samples.

Two kits are available which differ in the type of reductant supplied: sodium cyanoborohydride or non-toxic 2-picoline borane.

Traditional 2-AA and 2-AB labelling kits use sodium cyanoborohydride as a reducing agent during glycan labeling. This reagent is toxic so a fume cupboard should be used during handling. To conform with emerging health and safety regulations we are now replacing these with our new VP glycan kits that use picoline borane which is a significantly safer reductant.

LT-KAB-A2
2-Aminobenzamide (2-AB dye)
Dimethyl sulfoxide (DMSO)
Acetic acid
Sodium cyanoborohydride

LT-KAB-VP24
2-Aminobenzamide (2-AB dye)
Dimethyl sulfoxide (DMSO) / Acetic acid solution
2-picoline borane

Number of Samples One 2-AB labeling kit contains reagents to label up to 30 separate analytical samples per kit

Dye purity >99% by HPLC

Molecular weight 137

Lambda-ex 250 nm

Lambda-em 428 nm

Amount of Sample From 25 pmol up to 25 nmol glycans per sample.

Suitable Samples Any purified glycans with free reducing termini can be labeled.

Structural Integrity No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.

Labeling Efficiency Typically > 85 % (dependent on sample).

Labeling Selectivity Essentially stoichiometric labeling.