α(2-3) Sialidase Sp cleaves exclusively the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α(2-6) or α(2-8) linkages or on branched α(2-3) linkages. To cleave all non-reducing terminal sialic acid residues including branched sialic acids (linked to an internal residue) from complex carbohydrates and glycoproteins, use α(2-3,6,8,9) Sialidase Au (E-S001).

Source: Recombinant Streptococcus pneumoniae in E. coli

EC: 3.2.1.18

Alternate Names: Neuraminidase, N-acetylneuraminate glycohydrolase, Exo-α-sialidase

Contents:
Ludger Sialidase Sp - Kit Contents
Sialidase Sp in 50 mM sodium phosphate, pH 7.5
5x Reaction Buffer 250 mM sodium phosphate, pH 6.0

Specific Activity: = 250 U/mg
Activity: = 10 U/mL

Molecular weight: ~75,000 daltons

pH optimum 6.0

Suggested usage: 1. Add up to 100 µg of glycoprotein or 1 nmol of oligosaccharide to tube.
2. Add water to 14 µL
3. Add 4 µL 5X Reaction Buffer.
4. Add 2 µL α(2-3) Sialidase Sp.
5. Incubate at 37°C for 1 hour

Desialylation may be monitored by SDS-PAGE if the size differential between native and desialylated protein is sufficient for detection.

Specificity: Cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.

Specific Activity: Defined as the amount of enzyme required to produce 1 µmole of methylumbelliferone in 1 minute at 37°C, pH 5.0 from MU-NANA [2′-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid].

Storage Store enzyme at 4°C.