Alpha-MSH (Melanocyte Stimulating Hormone) Antibody / Alpha-MSH抗体/黑色素细胞刺激素抗体
The ImmunoStar alpha melanocyte stimulating hormone antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pituitary using biotin/avidin-HRP techniques.
Recommended primary dilution is 1/4000-1/6000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of alpha-MSH.
照片描述: IHC image of neurons staining for MSH in the rat hypothalamus (above) and rat pituitary with nickel preparation (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
宿主: Rabbit
规格/体积: 100 µL
性状: Lyophilized Whole Serum
反应性: Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Trout
别名: POMC; proopiomelanocortin, anti-Alpha-MSH
RRID: AB_572218
免疫原: alpha-MSH
Gene Symbol: POMC