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JaICA 8-OHdG ELISA试剂盒
2021-02-07 6432 分享到:

New 8-OHdG Check ELISA
8-oxo-dG/8-hydroxy-2'-deoxyguanosine             最畅销的8-OHdG ELISA试剂盒

DNA氧化生物标志物

新的JaICA 8-OHdG ELISA试剂盒,适用于对尿液样本进行氧化应激的评估。


关于8-羟基-2'-脱氧鸟苷(8-OHdG):

8ohdg_struct.jpg

8-羟基-2'-脱氧鸟苷(8-OHdG),又称为8-羟基脱氧鸟苷;8-hydroxy-2'-deoxyguanosine。


8-羟基-2-脱氧鸟苷(8-OHdG)是由羟基自由基、单线态氧和直接的光动力作用形成的DNA氧化损伤产物。8-OHdG可以在组织、血清、尿液和其它生物样品中检测。新的8-OHdG Check试剂盒是一种竞争性酶联免疫吸附测定(ELISA),利用对DNA损伤高度特异性的单克隆抗体(克隆#N45.1),与RNA氧化产物(例如8-羟基鸟嘌呤和8-羟基鸟苷)没有交叉反应。该试剂盒适用于从人和动物的尿液及其他生物样品中检测8-OHdG浓度。


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该8羟基脱氧鸟苷(8-OHdG) ELISA试剂盒利用抗8-羟基脱氧鸟苷(8-OHdG)单克隆抗体(克隆号N45.1)对8-OHdG的高度特异性识别原理设计。在进行操作前请阅读完整说明书或咨询北京中昊新生(zhscience.com)。


JaICA提供两种类型的8-OHdG ELISA试剂盒,具有不同的检测范围。新的8-OHdG ELISA试剂盒适用于人或动物的尿液和血清样品。如果需要检测人血清、组织、细胞培养样品的8-OHdG, 我们建议使用“高灵敏的8-羟基脱氧鸟苷ELISA试剂盒(货号 #KOG-HS10E)”。



技术参数:

检测原理:竞争法ELISA检测(450nm);

特异性:特异性识别8-OHdG,抗体已经过多种8-OHdG类似物的测试(guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G);

测定范围:0.5〜200ng/mL;

格式:96孔板(18个样品,3个平行对照);

应用:人和动物的尿液(对于血清和组织样本,建议使用高灵敏的8-OHdG Check ELISA试剂盒,货号 #KOG-HS10E);

储存:4 - 10°C(不能冷冻);

保质期:12个月(应在开启后2周内使用)。


试剂盒组份

WX20210207-153419.png



References

1)H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986)

[ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ]

2)R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)

[ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ]

3)S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997)

[ Specifity of the antibody clone N45.1.]

4)Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K: Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)

[ Development and assessment of 8-OHdG Check ELISA. ]

5)Nagayoshi Y, Kawano H, Hokamaki J, Miyamoto S, Kojima S, Shimomura H, Tsujita K, Sakamoto T, Yoshimura M, Ogawa H: Urinary 8-hydroxy-2'-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent cardiac events. Am J Cardiol. 95(4), p514-517 (2005)

[ Measurement of 8-OHdG in human urine. ]

6)Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA, Gonzalez-Gallego J: The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 95(5-6),p543-549(2005)

[ Study about exercise, antioxidant supplements and urinary 8-OHdG. ]

7)Someya T, Kaneko K, Yamada T, Yamashiro Y: Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats. Pediatr Nephrol 20(10),p1430-1434(2005)

[ Effect of antioxidants to 8-OHdG in rat urine. ]

8)Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K: Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage. J Neurol Sci 250(1-2),p159-161(2006)

[ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ]


日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒,并且处于该领域的领先地位。JaICA氧化应激相关产品:DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析,以及微量元素分析。详情请咨询JaICA中国区代理商:北京中昊新生科技(zhscience.com)。