17羟孕酮ELISA试剂盒,17-OHP ELISA试剂盒

多种物种

DetectX®17-Hydroxyprogesterone ELISA试剂盒定量检测各种样品和物种中的17-羟孕酮。

检测类型:Competitive ELISA

样本类型:Extracted Serum, Extracted Plasma, Urine, Fecal Extracts, Tissue Culture Media

灵敏度:20.3 pg/mL

适应物种:17HO-P在不同物种间是相同的

检测时间:1.5小时

样品数/板:40 in Duplicate

读数:Colorimetric, 450 nm

DetectX®17羟孕酮ELISA试剂盒用于定量检测提取的血清,提取的血浆,尿液,粪便提取物和组织培养样品中的17-羟孕酮。在检测之前,请阅读完整的试剂盒说明书或咨询中昊新生（zhscience）。

17-羟孕酮ELISA试剂盒提供有17-羟孕酮原液以生成标准曲线，和6,000到24.7 pg/mL的检测标准方案。将样品加入包被抗体的微孔板中以捕获绵羊抗体。 将17-羟基孕酮-过氧化物酶结合物添加到孔中，通过向每个孔中加入17-羟基孕酮多克隆抗体进行结合反应。孵育一个小时后，洗涤板并添加底物。底物与结合的17-羟基孕酮-过氧化物酶结合物反应。 短暂孵育后，停止反应，并在450nm处检测到所产生颜色的强度。

17-Hydroxyprogesterone is a steroid hormone belonging to the androgen group and is found and is in mammals, reptiles, birds, and other vertebrates. It was first isolated by Pfiffner and North in 1940. It is primarily produced in the adrenal glands but is also produced in the corpus luteum of the ovary. It is hydroxylated at the 11 and 21 positions to produce cortisol. A deficiency of either 11- or 21-hydroxylase results in decreased cortisol synthesis, and feedback inhibition of adrenocorticotropic hormone (ACTH) secretion is lost. Consequently increased pituitary release of ACTH will increase production of 17HO-P. However, if 17-alpha-hydroxylase or 3β-hydroxysteroid dehydrogenase type 2 is deficient, then 17HO-P levels will be low with either a possible increase in progesterone or pregnenolone respectively. Normal levels are 3-90 ng/dL in children. In women, normal levels are 20-100 ng/dL prior to ovulation, and 100-500 ng/dL during the luteal phase.

北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供17-羟孕酮ELISA试剂盒,17-OHP ELISA试剂盒最新现货优惠信息。