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KinaSorb™ 激酶(& ATP结合蛋白)富集试剂
货号:KE785-10 | 规格:10 preps | 价格:¥0.00 | 品牌:Biotech Support Group
北京中昊新生科技有限公司代理Biotech Support GroupKinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent激酶(与ATP结合蛋白)富集试剂。BSG专注蛋白质组学样品制备和富集,产品包括白蛋白清除剂,血红蛋白清除剂,DNA/RNA富集,糖蛋白富集,尿蛋白富集等。

KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent 激酶(与ATP结合蛋白)富集试剂

用于蛋白激酶和其他ATP结合蛋白的抽提富

KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent

Non-covalent immobilization of phosphate group with optimal nucleotide orientation & specificity

Enrichment 3-5X Kinase enrichment, protein recoverable, ~200 µg

60 minute, scaleable protocol compatible with functional assays, electrophoresis and LC-MS

Phosphatase activity & cyclic nucleotide phosphodiesterase (PDE) activity not detectable

Improves protein normalization when comparing heterogeneous tissues

On-bead digestion protocols for LC-MS annotation

KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. ATP – the common substrate for kinases, is reversibly immobilized with optimal orientation and specificity, to metallic oxide particles, producing a single-use highly efficient enrichment method for kinase & other ATP binding proteins. The standard prep protocol starts with 100 µl of clarified cellular extracts, or approximately 2 mg total cellular extract protein, but the process can be scaled up or down to accommodate different sample volumes and protein concentrations. The kit includes all necessary reagents for immediate use.


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