统一服务热线:400-888-0207 在线咨询 | 微信联系
首页 > 产品 > 产品类型 > 试剂 > 蛋白与脂质去除
KinaSorb™ 激酶(& ATP结合蛋白)富集试剂
货号:KE785-50 | 规格:50 preps | 价格:¥0.00 | 品牌:Biotech Support Group
北京中昊新生科技有限公司代理Biotech Support GroupKinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent激酶(与ATP结合蛋白)富集试剂。BSG专注蛋白质组学样品制备和富集,产品包括白蛋白清除剂,血红蛋白清除剂,DNA/RNA富集,糖蛋白富集,尿蛋白富集等。

KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent 激酶(与ATP结合蛋白)富集试剂

用于蛋白激酶和其他ATP结合蛋白的抽提富


KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent

Non-covalent immobilization of phosphate group with optimal nucleotide orientation & specificity

Enrichment 3-5X Kinase enrichment, protein recoverable, ~200 µg

60 minute, scaleable protocol compatible with functional assays, electrophoresis and LC-MS

Phosphatase activity & cyclic nucleotide phosphodiesterase (PDE) activity not detectable

Improves protein normalization when comparing heterogeneous tissues

On-bead digestion protocols for LC-MS annotation

KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. ATP – the common substrate for kinases, is reversibly immobilized with optimal orientation and specificity, to metallic oxide particles, producing a single-use highly efficient enrichment method for kinase & other ATP binding proteins. The standard prep protocol starts with 100 µl of clarified cellular extracts, or approximately 2 mg total cellular extract protein, but the process can be scaled up or down to accommodate different sample volumes and protein concentrations. The kit includes all necessary reagents for immediate use.




相关产品